Journal: Materials & Design

Article Title: Multifunctional nanofiber-based dressings in coordination with adipose-derived stem cells for accelerated burn wound healing

doi: 10.1016/j.matdes.2025.113929

Figure Lengend Snippet: Fig. 4. (A) Detection of ADSCs proliferation by ADM coating at different times. Quantification of (B) EGF and (C) FGF2 in ADSCs paracrine products. *** P < 0.001 representing a significant difference as compared with ADM (3 min) and TLWDA.

Article Snippet: Human EGF enzyme-linked immunosorbent assay (ELISA) kit and human FGF2 ELISA kit were purchased from BOSTER (Wuhan, China).

Techniques:

Journal: Biomedicines

Article Title: Hypoxia Induces DPSC Differentiation versus a Neurogenic Phenotype by the Paracrine Mechanism

doi: 10.3390/biomedicines10051056

Figure Lengend Snippet: Effects of hypoxia on the mRNA profile expression of DPSCs. ( A ) Real-time RT-qPCR analysis of the levels of expression of early and late markers of neural differentiation (nestin, GFAP, EGF, NGF, bFGF BDNF, GDNF) after 5 days and after 16 days of hypoxic exposure. ( B ) Secreted growth factors bFGF and EGF were detected in the conditioned media of DPSCs exposed or not exposed to hypoxia for 5 days and 16 days by human EGF and FGF basic ELISA Kits. Histograms indicate the means ± SE of three different cultures, each of which was tested in triplicate. Statistical analysis was performed by Student’s t -test; * p < 0.05; ** p < 0.01 vs. 5N.

Article Snippet: The quantity of EGF and bFGF, in each sample, was evaluated using the Human EGF ELISA Kit and the Human FGF basic ELISA Kit (Abcam, Cambridge, MA, USA), following the manufacturer’s instructions.

Techniques: Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

Journal: Advanced Science

Article Title: Gold Nanoturf‐Mediated Wireless Photothermal Upregulation of Human Adipose‐Derived Stem Cell Spheroids for Synergistic Skin‐Wound Closure

doi: 10.1002/advs.202515490

Figure Lengend Snippet: In vitro cell viability and gene/protein evaluation of photothermally upregulated hADSCs spheroids within the patch. a) Schematic illustrations of the formation and photothermal upregulation of hADSCs spheroids (D = 173 ± 14 µm; mean values ± s.d.; sample size = 90) within the patch under irradiation of the LED array (3 × 3, 630 nm). Inset schematics show the cross‐sectional view of hADSCs spheroids‐loaded patch along with the LED array inside the container filled with the culturing solution. b) Representative results of live (fluorescein diacetate (FDA); green) and dead (ethidium bromide (EB); red) assay of no treatment (NT), photothermal stimulation for 1 h (PT 1H), and 24 h (PT 24H) groups. Scale bar is 500 µm. c) Cell viability evaluated with flow cytometry double staining of Annexin V and 7‐Aminoactinomycin D (7‐AAD) (Sample size = 4). d) Comparison of gene expressions in NT, PT 1H, and PT 24H group evaluated by quantitative reverse transcription PCR (qRT‐PCR) (Sample size = 4, one‐way ANOVA, ** P< 0.01, *** P< 0.001 compared to NT, $ P < 0.05, $$ P < 0.01, and $$$ P < 0.001 compared to each group). Evaluated genes are associated with factors of heat shock protein (HSP), angiogenesis, proliferation, and anti‐inflammation. e–h) Amount of vascular endothelial growth factor (VEGF; e), keratinocyte growth factor (KGF; f), fibroblast growth factor (FGF2; g), hepatocyte growth factor (HGF; h) secreted from hADSCs spheroids in each group as evaluated by enzyme‐linked immunosorbent assay (ELISA, Sample size = 6, one‐way ANOVA).

Article Snippet: To analyze the secretion of paracrine factors in CM from each group, ELISA kits were used for human VEGF, HGF, KGF, and FGF2 (R&D Systems), according to the manufacturer's instructions.

Techniques: In Vitro, Irradiation, Flow Cytometry, Double Staining, Comparison, Reverse Transcription, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay